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supersignal west pico plus ecl kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher supersignal west pico plus ecl kit
    Supersignal West Pico Plus Ecl Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+supersignal+west+pico+kit/pm39814017-269-14-20
    Average 90 stars, based on 1 article reviews
    supersignal west pico plus ecl kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Membrane:

    Article Title: The impact of di-2-ethylhexyl phthalate on sperm fertility
    Article Snippet: The membrane was then washed three times in PBST and probed with a secondary horseradish peroxidase-conjugated antibody (Abcam) at a dilution of 1:15,000 in 1x PBST. .. After subsequent washing, the membrane was developed with an ECL SuperSignal West Pico kit (Pierce). .. After detection, the membrane was stripped and re-probed with mouse tubulin-alpha ab-2 (Sigma-Aldrich), 1:5000 dilution.

    Article Title: The Impact of Di-2-Ethylhexyl Phthalate on Sperm Fertility.
    Article Snippet: The membrane was then washed three times in PBST and probed with a secondary horseradish peroxidaseconjugated antibody (Abcam) at a dilution of 1:15,000 in 1× PBST. .. After subsequent washing, the membrane was developed with an ECL SuperSignal West Pico kit (Pierce) according to the manufacturer’s instructions. .. After detection, the membrane was stripped and re-probed with mouse tubulin-alpha ab2 (Sigma-Aldrich), 1:5000 dilution.

    Article Title: The Impact of Di-2-Ethylhexyl Phthalate on Sperm Fertility
    Article Snippet: The membrane was then washed three times in PBST and probed with a secondary horseradish peroxidase-conjugated antibody (Abcam) at a dilution of 1:15,000 in 1× PBST. .. After subsequent washing, the membrane was developed with an ECL SuperSignal West Pico kit (Pierce) according to the manufacturer’s instructions. .. After detection, the membrane was stripped and re-probed with mouse tubulin-alpha ab-2 (Sigma-Aldrich), 1:5000 dilution.

    Incubation:

    Article Title: miR-17-5p is highly expressed in patients with lung cancer and promoted lung cancer by targeting SIK1.
    Article Snippet: The membranes were blocked for 1 h, and incubated with SIK1 monoclonal antibody (Abclonal, Cat# ABIN716411, dilution 1:1000, Wuhan, China) and β-actin mouse anti-human monoclonal antibody (Abcam, Cat# ab8226, dilution 1:1000, Cambridge, MA, USA) overnight at 4 °C. .. Next, the membranes were incubated with secondary antibody and the ECL SuperSignal West Pico Kit (ThermoFisher, Waltham, USA) was used for protein imaging and photographic documentation. ..

    Imaging:

    Article Title: miR-17-5p is highly expressed in patients with lung cancer and promoted lung cancer by targeting SIK1.
    Article Snippet: The membranes were blocked for 1 h, and incubated with SIK1 monoclonal antibody (Abclonal, Cat# ABIN716411, dilution 1:1000, Wuhan, China) and β-actin mouse anti-human monoclonal antibody (Abcam, Cat# ab8226, dilution 1:1000, Cambridge, MA, USA) overnight at 4 °C. .. Next, the membranes were incubated with secondary antibody and the ECL SuperSignal West Pico Kit (ThermoFisher, Waltham, USA) was used for protein imaging and photographic documentation. ..



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    Reagents and tools table

    Journal: The EMBO Journal

    Article Title: Molecular mechanism targeting condensin for chromosome condensation

    doi: 10.1038/s44318-024-00336-6

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: ECL SuperSignal West Pico chemiluminescence kit , Thermo Fisher Scientific , Cat#34580.

    Techniques: Recombinant, Silver Staining, Software